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Image Search Results


Journal: eLife

Article Title: ONC201/TIC10 enhances durability of mTOR inhibitor everolimus in metastatic ER+ breast cancer

doi: 10.7554/eLife.85898

Figure Lengend Snippet:

Article Snippet: Membranes were immunoblotted overnight with antibodies against BiP (C50B12) (#3177, RRID: AB_2119845 ), pFoxO3a (Ser294) (#5538, RRID: AB_10696878 ), FoxO3a (D19A7) (#12829, RRID: AB_2636990 ), pERK1/2 Thr202/Tyr204 (197G2) (#4377, RRID: AB_331775 ), ERK1/2 (137F5) (#4695, RRID: AB_390779 ), pAKT Ser473 (D9E) (#4060, RRID: AB_2315049 ), AKT (C67E7) (#4691, RRID: AB_915783 ), pS6Ser240/244 Ribosomal Protein (D68F8) (#5364, RRID: AB_10694233 ), S6 Ribosomal Protein (54D2) (#2317, RRID: AB_2238583 ), β-actin (8H10D10) (#12262, RRID: AB_2566811 ), β-actin (13E5) (#5125, RRID: AB_1903890 ) from Cell Signaling Technology, CREB-2/ATF-4 (B-3) (sc-390063, RRID: AB_2810998 ), mtTFA (TFAM) (C-9) (sc-376672, RRID: AB_11150497 ) from Santa Cruz Biotechnology, TUFM (#PA5-27511, RRID: AB_2544987 ) and TRAIL (#MA1-41027, RRID: AB_1087999 ) from Thermo Fisher Scientific and CHOP (Cat#15204-1-AP, RRID: AB_2292610 ) from Proteintech.

Techniques: Transfection, Construct, Cell Viability Assay, Viability Assay, Software

Primary antibodies:

Journal: Human Molecular Genetics

Article Title: Prevention of premature death and seizures in a Depdc5 mouse epilepsy model through inhibition of mTORC1

doi: 10.1093/hmg/ddaa068

Figure Lengend Snippet: Primary antibodies:

Article Snippet: Antibody Species Dilution Vendor/Manufacturer Catalog Number anti-beta-actin Rabbit 1:2000 (WB) Cell Signaling Technology #4967 anti-beta-actin Mouse 1:2000 (WB) Sigma #A5441 anti-phospho-Akt (Ser473) Rabbit 1:1000 (WB) Cell Signaling Technology #4060 anti-phospho-4E-BP1 (Thr37/46) Rabbit 1:250 (WB) Cell Signaling Technology #2855 anti-4E-BP1 (clone 53H11) Rabbit 1:500 (WB) Cell Signaling Technology #9644 anti-pan-Akt (clone 40D4) Mouse 1:1000 (WB) Cell Signaling Technology #2920S anti-phospho-S6 (Ser235/236) Rabbit 1:1000 (WB) Cell Signaling Technology #2211 anti-phospho-S6 (Ser240/244) Rabbit 1:1000–1:2000 (WB); 1:800 (IF) Cell Signaling Technology #5364 anti-S6 (clone 54D2) Mouse 1:500 (WB) Cell Signaling Technology #2317S anti-NPRL2 Rabbit 1:1000 (WB) Cell Signaling Technology #37344S anti-GFAP Mouse 1:1000 (WB); 1:300 (IF) Cell Signaling Technology #3670 anti-NeuN Mouse 1:1000 (IF) Abcam #ab104224 anti-Cux1 Rabbit 1:100 (IF) Santa Cruz #SC-13024 Open in a separate window Primary antibodies: Primary mouse astrocyte cultures Primary mixed glial cultures were prepared from P0 to P2 mouse brains with modification of published protocols ( 33 , 42 ).

Techniques: